Effect of adiponectin on colony formation of colony-forming units—granulocyte-macrophage (CFU-GM) from human bone marrow mononuclear cells (BMMNC).
(A) Human BMMNC (5 × 104 cells) were cultured in serum-free methylcellulose medium containing a combination of growth factors with the indicated concentrations of adiponectin (0-50 μg/mL), and CFU-GM colony formation was estimated. (B) Purified antiadiponectin MoAb (MoAb 9104) or purified murine IgG1(30 μg/mL) was added to the methylcellulose culture containing a combination of growth factors with or without 10 μg/mL adiponectin, and CFU-GM colony formation from 5 × 104 human BMMNC was assessed. The data shown are the mean ± SD percentages of inhibition in 3 independent experiments in which each experiment was set up with quadruple cultures. Control (medium alone) colony numbers ranged from 54 to 77. Significant differences from control values are indicated by 1 (P < .05) or 2 (P < .01) asterisks.
