Effects of adiponectin on numbers of colonies.
(A) Human BMMNC (5 × 104 cells) were cultured in serum-free methylcellulose medium with a combination of growth factors containing 10 μg/mL adiponectin, human serum albumin (HSA), or medium alone, and colony formations from CFU-GM, colony-forming units—macrophage (CFU-M), burst-forming units—erythroid (BFU-E) and colony-forming units—mixed erythroid-myeloid (CFU-mixed) were evaluated. Control colony numbers (medium alone) ranged from 31 to 60 for CFU-GM, 4 to 13 for CFU-M, 62 to 99 for BFU-E, and 3 to 6 for CFU-mixed. (B) Colony formations by CFU-GM stimulated with recombinant human (rh) granulocyte-macrophage colony-stimulating factor (GM-CSF) alone, CFU-M stimulated with rh macrophage colony-stimulating factor (M-CSF) alone, and colony-forming units—granulocyte (CFU-G) stimulated with rh granulocyte colony-stimulation factor (G-CSF) alone from human BMMNC (1 × 105 cells) were evaluated in the absence or presence of 10 μg/mL adiponectin. Control (HSA) colony numbers ranged from 98 to 114 for CFU-GM, 73 to 86 for CFU-M, and 67 to 76 for CFU-G. The data shown are the mean ± SD percentages of control values from 3 independent experiments in which each experiment was set up with quadruple cultures. Significant differences from control values are indicated by 1 (P < .05) or 2 (P < .01) asterisks.
