Effect of adiponectin on colony formation from human CD34⁺ hematopoietic progenitors.

(A) Human CD34⁺ cells (500 cells) were cultured in serum-free methylcellulose medium with a combination of growth factors containing 10 μg/mL adiponectin, HSA, or medium alone, and colony formations from CFU-GM, BFU-E, and CFU-mixed were evaluated. Control (medium alone) colony numbers ranged from 28 to 47 for CFU-GM, 53 to 109 for BFU-E, and 14 to 22 for CFU-mixed. (B) Human CD34⁺cells (500 cells) were cultured in serum-free methylcellulose medium with a combination of growth factors containing 10 μg/mL C1q, adiponectin, or HSA, and CFU-GM colony formation was estimated. Control (HSA) colony numbers ranged from 49 to 70. (C) Single human CD34⁺ cells were placed in 96-well flat-bottomed plates (500 wells). The cells in each well were cultured with serum-free methylcellulose medium with a combination of growth factors containing 10 μg/mL adiponectin or HSA, and CFU-GM colony formation was estimated. Control (HSA) colony numbers ranged from 40 to 79. The data shown are the mean ± SD percentages of control values from 3 independent experiments.