Adiponectin inhibits the growth of an acute myelomonocytic cell line, M1, by inducing apoptosis.
(A) Triplicate aliquots of M1 cells (1 × 104 cells) were incubated in serum-free medium containing 10 μg/mL adiponectin, HSA, or medium alone for the indicated times. At various times after initiation of the culture, viable cell numbers (upper panel) and viability (lower panel) of M1 cells were determined by trypan blue dye exclusion. The results are shown as the mean ± SD values from triplicate cultures. (B-D) M1 cells were incubated in serum-free medium containing 10 μg/mL adiponectin or HSA for the indicated times and subjected to morphologic (B), nuclear DNA content (C), and DNA fragmentation (D) analyses. Each figure shows 1 of 3 similar experiments.
