Effect of adiponectin on the phagocytic activity of macrophages.

Human macrophages were incubated in serum-free medium containing 10 μg/mL adiponectin, HSA, or medium alone for 24 hours and subjected to phagocytosis assay. The fluorescence intensity level of nonspecifically binding microspheres was determined by using macrophages treated with 0.1% sodium azide on ice during incubation with fluorescent microspheres. The data represent the mean ± SD values from triplicate cultures. Significant differences from control (HSA) values are indicated by 2 (P < .01) asterisks. Similar results were obtained in 3 independent experiments.