Effects of adiponectin on expression of proinflammatory cytokines in macrophages stimulated with lipopolysaccharide (LPS).

(A) After preincubation with 10 μg/mL adiponectin, HSA, or medium alone for 24 hours, human macrophages were stimulated with 10 μg/mL LPS for 4 hours. Total RNAs were then isolated and subjected to Northern blot analyses using the cDNAs of tumor necrosis factor α (TNF-α), interleukin-1 β (IL-1β), interleukin-6 (IL-6), and Chinese hamster ovary (CHO) cells as probes. This figure shows 1 of 3 similar experiments. (B) After preincubation with 10 μg/mL adiponectin or HSA for 24 hours, human macrophages were stimulated with 10 μg/mL LPS for the indicated periods. (C) After preincubation with 10 μg/mL adiponectin or HSA for the indicated periods, human macrophages were stimulated with 10 μg/mL LPS for 4 hours. The expression of TNF-α and CHO genes in the indicated cells was determined by Northern blot analyses. Each figure in B and C shows 1 of 2 independent experiments.