Fig. 7.
GR-induced apoptosis in NK-cells.
One hundred thousand NK cell-enriched lymphocytes were admixed with 2.5 to 5 × 104 GR for 16 hours. The cell cultures were treated with culture medium (○), histamine (10 μmol/L, ●), or histamine + ranitidine (10 μmol/L + 10 μmol/L, ▴). After incubation, cells were labeled with anti-CD56 and assayed for morphologic apoptosis using flow cytometry. Data are the percentage of apoptotic NK cells among all gated lymphocytes and are the mean ± SEM of 5 blood donors. The inset shows the corresponding data for apoptotic T cells (CD3+/CD56−) and are the mean ± SEM of 5 donors.
