Fig. 2.
Induction of apoptosis in HUVECs treated with As2O3.
Early passage, rapidly proliferating HUVECs were incubated with different concentrations of As2O3, dual-stained with Annexin-V–FITC and PI and analyzed by flow cytometry. After 72 hours, there was a dose-dependent increase in early and late apoptotic cells, as shown in the second and fourth quadrants of the plots. Results are representative of 3 similar independent experiments.
