Western blot (nonreduced) of A antigen on GPs in a lysate prepared from Type II A1 high-expresser platelets with and without absorption with mAbs MBC 132.1 and MBC 78.2.
(Lane 1) After absorption with MBC 132.1 (anti-GPIIb), the higher molecular weight band was removed. (Lane 2) Without absorption, 2 prominent bands with mobilities expected of GPIIb and PECAM were identified. (Lane 3) The lower molecular weight band was removed by absorption with MBC 78.2 (anti-PECAM). (Lane 4) Both bands were removed by combined absorption with the 2 mAbs. (Lanes 5 and 6) Recovery of the expected bands upon elution from the mAb-conjugated beads used for absorption in lanes 1 and 3. Similar results were obtained with platelets from a second Type II high expresser of A1(not shown).
