IL-7 induces OC formation in monocytes via actions on T cells.

Purified monocytes alone (■), monocytes with T cells replaced (▪), and monocytes with B cells replaced (░) were cultured with or without rhIL-7 (2.5 ng/mL) or CM from purified T cells (▨) cultured in the presence or absence of rhIL-7. After 7 to 10 days, TRAP⁺ multinucleated cells were counted. Only IL-7–stimulated T-cell replete cultures and IL-7–stimulated T-cell CM were able to stimulate appreciable OC formation. Data points are shown as average + SD of 3 replicate wells. Data are representative of 3 independent experiments.