Transduction of fresh acute lymphoblastic leukemia (ALL) cells and acute myeloid leukemia (AML) cells with pHR-GM/CD.

The panels on the left represent the nontransduced (mock-transduced) cells, and the panels on the right show the cells transduced with the pHR-GM/CD vector. Dot-plot analysis of the double staining for lineage markers (fluorescein isothiocyanate–conjugated anti-CD10 and anti-CD33) and transgene expression (phosphatidylethanolamine-conjugated anti-CD80) is shown. Isotype nonspecific control antibodies were used to set up the background levels.