Fig. 3.
Fig. 3. Flow cytometric analysis of peritoneal cells. / Cells from wt (A,C,E,G,I) and Lsp1−/−(B,D,F,H,J) mice were stained with anti-Mac-1-FITC versus anti-CD5-PE (A,B) or anti-F4/80-biotin/streptavidin-PE (C,D). The inset in panel D shows histograms of F4/80 stainings from panels C (regular line) and D (bold line). Panels E, F, G, and H illustrate the FSC/SSC plots of all cells (E,F) and of Mac-1hiCD5− subsets (G,H) gated from the analysis illustrated in panels A and B, respectively. Peritoneal cells with FSC/SSC properties of small live lymphocytes were analyzed for staining with anti-B220-FITC versus anti-CD5-PE (I,J).

Flow cytometric analysis of peritoneal cells.

Cells from wt (A,C,E,G,I) and Lsp1−/−(B,D,F,H,J) mice were stained with anti-Mac-1-FITC versus anti-CD5-PE (A,B) or anti-F4/80-biotin/streptavidin-PE (C,D). The inset in panel D shows histograms of F4/80 stainings from panels C (regular line) and D (bold line). Panels E, F, G, and H illustrate the FSC/SSC plots of all cells (E,F) and of Mac-1hiCD5− subsets (G,H) gated from the analysis illustrated in panels A and B, respectively. Peritoneal cells with FSC/SSC properties of small live lymphocytes were analyzed for staining with anti-B220-FITC versus anti-CD5-PE (I,J).

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal