Recovery and adhesion of peritoneal macrophages.

(A) The effect of EDTA on the recovery of peritoneal macrophages. The proportion of Mac-1^hiCD5⁻ macrophages harvested from the peritoneum of wt (+/+) orLsp1^−/−(−/−) mice with or without 5 mmol/L EDTA in the lavage buffer are shown. Data are presented as mean values ± 1 SEM from 3 mice per genotype (+EDTA) and 8 or 9 mice (wt orLsp1^−/−, respectively, −EDTA). *,P = .008, **, P = .0003. (B) Adhesion of the peritoneal macrophages to fibronectin in vitro. F4/80⁺macrophages from wt and Lsp1^−/−mice adhered to fibronectin-coated coverslips were counted within a defined area as described in “Materials and methods.” Data are presented as means ± 1 SEM from 4 mice per genotype.