Fig. 8.
Chemotaxis of wt (+/+) and LSP1 −(−/−) neutrophils.
HBSS containing Ca++ and Mg++ with 0.1% BSA in the absence (0) or presence of fMLP (10−6-10−4 mol/L) or KC (10−6mol/L) was placed in the bottom chambers and calcein-am-labeled cells (4 × 106/mL) were loaded in the upper wells and chemotaxis assay was performed as described in “Materials and methods.” The number of cells migrating across polycarbonate filters ± 1 SEM of 6 mice per genotype is represented. *, P < .01.