Effect of HIV-1ΔEN V3 transduction on large particle uptake by immature DCs.

Immature DCs were transduced by HIV-1ΔEN V3 as described in the legend to Figure 2. Transduced as well as untransduced DCs were incubated with red fluorescent latex beads and analyzed after varying lengths of time by FACS. Background due to nonspecific binding of latex beads to DCs was determined by incubating sodium azide-treated DCs with latex beads. This value was subtracted from the data shown. Mean ± SD of at least 2 experiments are shown.