Fig. 5.
Effect of HIV-1ΔEN V3 transduction on T-cell stimulation by mature DCs.
DCs were transduced and maturated as described in legends to Figure 2and Figure 3, respectively, and subsequently irradiated. Graded numbers of transduced and sorted (GFP+: more than or equal to 97%) or untransduced DCs were then incubated with 1 × 105allogenic peripheral blood leukocytes in the mixed leukocyte reaction. T-cell proliferation was determined after 5 days by [3H]-thymidine incorporation. Mean ± SD from 2 experiments are shown.