Immunofluorescence microscopy of human spleen sections.

(A and B) Paraffin sections of Gaucher spleen used in the subtraction procedure stained with CD68 monoclonal antibody detected by an immunoperoxidase method to show pathologic macrophages (panel A, original magnification × 50; panel B, original magnification × 400). (C and E) Cathepsin B immunofluoresence in sections of 2 different independent Gaucher spleens (original magnification × 400). (G and H) Nonimmune control fluorescence in Gaucher spleens (original magnification × 400). (D and F) Cathepsin K immunofluorescence in 2 Gaucher spleen sections (panel D, original magnification × 100; panel F, original magnification × 400).