Double-color flow cytometric analysis of the kinetics of CXCR3 expression on human cord blood CD34⁺ hematopoietic progenitors.

The cells were either freshly isolated (A) or stimulated with GM-CSF (10 ng/mL) for 6 hours (B), 16 hours (C), 24 hours (D), 36 hours (E), or 48 hours (F). The cells were then stained with fluorescein isothiocyanate, conjugated (FITC)–labeled anti-CXCR3 monoclonal antibody (mAb). The data are from a single experiment representative of 2 similar experiments performed.