Adhesion of CD34⁺ hematopoietic progenitors from human cord blood induced by γIP-10 (indicated by 1), Mig (2), or SDF-1α (3).

The CD34⁺ progenitor cells were either freshly isolated (A), stimulated with GM-CSF (B), or stimulated with GM-CSF and incubated with anti-CXCR3 mAb (C). The cells were either freshly isolated from cord blood from a normal birth, preincubated with GM-CSF for 36 hours only, or preincubated with GM-CSF and then incubated with CXCR3 mAb (5 μg/mL) for 60 minutes at room temperature before the adhesion assay. All chemokines were applied at a concentration of 100 ng/mL. The data represent mean values from at least 3 experiments performed. Results are expressed as the mean ± SD percentage of adherent cells and are based on triplicate determinations of adhesion for each chemokine used.