Double-color flow cytometric analysis of the distribution of different integrins on CD34⁺ hematopoietic progenitors.

The CD34⁺ cells were either stimulated with GM-CSF for 36 hours (A-G) or stimulated with GM-CSF and then with γIP-10 for 8 hours (H-N). The cells were isolated from cord blood from a normal birth and either preincubated with GM-CSF (10 ng/mL) for 36 hours only or preincubated with GM-CSF and then stimulated with γIP-10 (100 ng/mL) for 8 hours. Subsequently, the cells were stained with the indicated anti-integrin mAbs. The data are from a single experiment representative of 4 similar experiments performed.