Adhesion of CD34⁺ hematopoietic progenitors from human cord blood.

Adhesion induced by γIP-10 (A), Mig (B), and SDF-1α (C) is shown. The cells were either stimulated with GM-CSF alone (indicated by 1) or stimulated with GM-CSF and then incubated with anti-CD49a mAb (2), anti-CD49b mAb (3), anti-CD49a and anti-CD49b mAbs (4), or isotype IgG1 (5; control for a-CD49a and a-CD49b). The cells were preincubated with different mAbs (5 μg/mL) for 60 minutes at room temperature before the adhesion assay. All chemokines were applied at a concentration of 100 ng/mL The data represent mean values from at least 3 experiments performed. Results are expressed as the mean ± SD percentage of adherent cells and are based on a triplicate determination of adhesion for each antibody used.