Assessment of cellular distribution of CXCR3 after stimulation.

(A) CXCR3 distribution on GM-CSF–stimulated CD34⁺hematopoietic progenitors before the chemotaxis assay. After stimulation, the CXCR3 receptors redistributed to the leading edge of the polarized migrating GM-CSF–stimulated CD34⁺hematopoietic progenitors, toward γIP-10 (B), Mig (C), or SDF-1α (D). The CD34⁺ cells were stimulated with GM-CSF for 36 hours and then subjected to the chemotaxis assay. The migrating cells were collected and stained and then photographed under epifluorescence conditions (original magnification ×1200; bar, 12 μm).