Day-course study of Pyk2 and Syk induction in HL-60 cells in the presence of dimethyl sulfoxide (DMSO).

HL-60 cells were treated with 1.25% DMSO for the indicated days, and whole cell lysates were subjected to immunoblotting analysis with anti-Pyk2 (A) or anti-Syk (B) polyclonal antibody. In each lane, the equivalent amounts of protein (8.5 μg) from whole cell lysates were loaded. In each panel, the positions of molecular markers are shown on the left in kilodaltons. Arrows indicate the position of a Pyk2 (A) or Syk (B) protein band.