Reconstitution of LCs and DDCs from BM cells.

BM cells (2 × 10⁶) from Ly 5.2 (A, B, D) or Ly 5.1 (C) mice were injected IV into 7 Gy γ-irradiated Ly 5.1 recipient mice. Epidermal LCs and DDCs were isolated at the indicated times from the ear dorsal and ventral halves after separation of the epidermis from the dermis by trypsin treatment. The epidermal or dermal sheets were then cultured for 12 hours with GM-CSF, and LCs and DDCs were collected and analyzed for donor-derived Ly 5.2⁺ cells. Mice in panels B, C, and D were exposed to UV-C irradiation for 30 minutes, 24 hours after transfer of BM cells. The percentage of Ly 5.2⁺and Ly 5.2⁻ MHC II⁺ LCs is indicated. These results are representative of 4 experiments with similar results.