Reconstitution of LCs and DDCs from CD4^lowprecursors.

Thymic CD4^low precursors (3 × 10⁴) were injected IV into 7 Gy γ-irradiated 8-week-old C57 BL/6 Ly 5.1 Pep^3b recipient mice, along with 4 × 10⁴ Ly 5.1 BM cells to ensure survival of recipients. Mice were exposed to UV-C irradiation for 30 minutes, 24 hours after transfer of CD4^low precursors. Control mice were injected with 4 × 10⁴ Ly 5.1 BM cells. At the indicated times mice were analyzed for donor-derived Ly 5.2 LCs or DDCs. The phenotype of Ly 5.2⁺ LCs reconstituted from CD4^low precursors 2 weeks after transfer is shown. The percentage of Ly 5.2⁺and Ly 5.2⁻ MHC II⁺ LCs and DDCs is indicated. These results are representative of 3 experiments with similar results.