Fig. 2.
Fig. 2. Chemotherapy-induced apoptosis of JM-1 cells is delayed by coculture with stromal cells. / After 48 and 72 hours of culture in 0.1 μg/mL Ara-C (A, B) or 5 μmol/L VP-16 (C, D), JM-1 cells were collected as described and stained with Annexin-V–FITC. Samples included untreated JM-1 control cells (control), JM-1 cells treated on an adherent stromal cell layer (stroma), and JM-1 cells treated in medium alone (no stroma). JM-1 cells were collected on a Becton Dickinson Flow Cytometer, and data were analyzed using CellQuest software.

Chemotherapy-induced apoptosis of JM-1 cells is delayed by coculture with stromal cells.

After 48 and 72 hours of culture in 0.1 μg/mL Ara-C (A, B) or 5 μmol/L VP-16 (C, D), JM-1 cells were collected as described and stained with Annexin-V–FITC. Samples included untreated JM-1 control cells (control), JM-1 cells treated on an adherent stromal cell layer (stroma), and JM-1 cells treated in medium alone (no stroma). JM-1 cells were collected on a Becton Dickinson Flow Cytometer, and data were analyzed using CellQuest software.

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