Effect of hIL-10 on the expression of CD16 and CD64 on monocytes/macrophages within PBMC.

PBMC were cultured in growth medium alone or in the presence of increasing concentrations of hIL-10 for 72 hours. Cells were stained with (A) CD64 or (B) CD16 mouse antihuman monoclonal antibodies, and the monocyte/macrophage population within PBMC was gated out on the basis of forward- and side-scatter. CD16 and CD64 expression on monocytes were determined by FACS and are presented as mean fluorescence intensity ± 2 SD. Data are representative of 3 separate experiments.