Effect of variably desulfated heparins on the hIL-10–induced expression of CD16 on monocytes/macrophages.

PBMC were stimulated with hIL-10 in the presence or absence of increasing concentrations of heparin (●),N-acetyl-de-O-sulfated heparin (♦), de-N-sulfated heparin (▴), de-O-sulfated heparin (○) for 72 hours. After the incubation period, CD16 expression on monocytes/macrophages was determined as described in Figure 3. Results are expressed as percentage inhibition of CD16 induced by hIL-10 ± 2 SD. Data are representative of 2 separate experiments.