Effect of sodium chlorate on the ability of hIL-10 or TNF-α to up-regulate the expression of CD16 or ICAM-1 on monocytes/macrophages.
PBMC were grown in the presence or absence of sodium chlorate for 24 hours. (A) hIL-10 (10 ng/mL) or (B) TNF-α (20 ng/mL) was added in chlorate-containing medium for an additional 48 hours. In some experiments, MgSO4 (15 mmol/L) was added together with NaClO3 and hIL-10. After the incubation period, the cells were stained with (A) CD16 or (B) ICAM-1 mouse antihuman monoclonal antibodies. The monocyte/macrophage population within PBMC was gated out on the basis of forward- and side-scatter. CD16 or ICAM-1 expression on monocytes from quadruplicate cultures was determined by FACS and is presented as percentage control or mean fluorescence intensity ± 4 SD. Data are representative of 2 separate experiments.
