Detection of WT1 overexpression in human leukemias by real-time PCR.

Total mRNA from BM, PBMC, cell lines, and human leukemias were reverse transcribed to cDNA, and WT1 gene expression was determined by semiquantitative real-time RT-PCR. Results are expressed relative to normal PBMC. Mean values of triplicates are depicted. PBMC and BM values represent the means of 3 different samples derived from healthy human donors. K562, human leukemia, chronic myeloid leukemia; U937, human lymphoma; > 95% CD34⁺, more than 95% pure CD34⁺ cells; BM, human bone marrow; PBMC, human PBMC; CD15⁺, more than 90% pure CD15⁺ cells; AML 1 through AML 9, bone marrow from patients with AML at the time of diagnosis.