Detection of WT1 overexpression in murine malignancies by real-time PCR.

Total mRNA from murine cancers and leukemia cell lines was reverse transcribed to cDNA, and WT1 gene expression was determined by semiquantitative real-time RT-PCR. Results are expressed relative to normal murine PBMC. Mean values of triplicates are depicted. PBMC and BM values represent the means of 3 different samples derived from normal B6 mice. TRAMP-C, SV40-transformed prostate; BM, murine BM; PBMC, murine PBMC; EL-4, carcinogen-induced thymoma; LSTRA, MuLV-induced leukemia; BLKSV40 HD2, SV40-transformed fibroblast; S49.1, lymphoma, T-cell-like; MethA, sarcoma; P3.6.2.8.1, myeloma.