Detection of WT1-specific antibodies in B6 mice immunized with WT1 peptides.

Three weeks after the third immunization with vaccine A, sera were obtained. Lanes 1, 3, and 5 show the molecular weight marker (MWM); lanes 2, 4, and 6 show the WT1 polypeptide WT N180 (180 amino acids in length, mapping within the amino terminus domain of the WT1 protein). The primary antibodies used were WT180 in lane 2, sera of nonimmunized B6 mice in lane 4, and sera of B6 mice immunized with the vaccine A in lane 6.