Detection of WT1-specific antibodies in B6 mice immunized with the WT1-positive tumor cell line TRAMP-C.

Mice were inoculated twice at 2-week intervals by subcutaneous injection with WT1 overexpressing TRAMP-C cells. Three weeks after the final immunization, sera were obtained. Lanes 1, 3, and 5 show the molecular weight marker (MWM); lanes 2, 4, and 6 show the WT1 polypeptide WT N180. Primary antibodies used were WT180 in lane 2, sera of nonimmunized B6 mice in lane 4, and sera of B6 mice immunized with TRAMP-C tumor cells in lane 6.