Organ leukocyte accumulation is augmented in DARC nullizygous mice.

Mild leukocytic infiltrates were observed in lung and liver sections from DARC^+/+ mice (panels A and D, respectively). Accumulation of leukocytes was markedly increased in lungs and livers from DARC^−/− mice (panels B and E, respectively). Neutrophil accumulation, as measured by tissue MPO content, was significantly greater in DARC^−/− mice in lung (panel C) and liver (panel F), compared with DARC^+/+ mice (*P < .05). All sections were stained with hematoxylin and eosin; original magnification: A-E, 4×; insets, 20×. For analysis of tissue MPO content, bars represent mean ± SEM with n = 5 per group.