American Society of Hematology

Fig. 3.

$Fig. 3. Effects of IL-3 and IL-11 on the in vivo reconstituting activity of KL+FL+MGDF-expanded Lin−Sca-1+c-kit+ cells. / Either immediately after isolation (day 0) or after cytokine-induced ex vivo expansion, 500 to 750 freshly isolated LSK Ly5.1+cells or their expansion equivalent were transplanted, together with 150 000 to 200 000 unfractionated Ly5.2+ BM cells, into lethally irradiated (Ly5.2+) recipients. Peripheral blood cells were analyzed by flow cytometry 6 weeks (A) or 4 months (B) after transplantation for donor (Ly5.1) and competitor/recipient (Ly5.2) origin. All data were derived from 4 experiments, with a total of 11 to 13 recipients per culture condition and time point, except for KFM at 240 hours, derived from 2 experiments with 7 recipients per culture condition and time point. Reconstitution levels (relative to the mean of that obtained with freshly isolated LSK cells within each individual experiment) are shown ± SEM. The mean reconstitution levels in mice transplanted with uncultured LSK cells were: 6 weeks, 50% (experiment 1), 35% (experiment 2), 17% (experiment 3), 40% (experiment 4); 4 months, 41% (experiment 1), 34% (experiment 2), 22% (experiment 3), and 40% (experiment 4). No differences in lineage distribution of donor-derived cells at 4 months were found between freshly isolated and cultured cells (see Table 2 for details).$

Effects of IL-3 and IL-11 on the in vivo reconstituting activity of KL+FL+MGDF-expanded Lin⁻Sca-1⁺c-kit⁺ cells.

Either immediately after isolation (day 0) or after cytokine-induced ex vivo expansion, 500 to 750 freshly isolated LSK Ly5.1⁺cells or their expansion equivalent were transplanted, together with 150 000 to 200 000 unfractionated Ly5.2⁺ BM cells, into lethally irradiated (Ly5.2⁺) recipients. Peripheral blood cells were analyzed by flow cytometry 6 weeks (A) or 4 months (B) after transplantation for donor (Ly5.1) and competitor/recipient (Ly5.2) origin. All data were derived from 4 experiments, with a total of 11 to 13 recipients per culture condition and time point, except for KFM at 240 hours, derived from 2 experiments with 7 recipients per culture condition and time point. Reconstitution levels (relative to the mean of that obtained with freshly isolated LSK cells within each individual experiment) are shown ± SEM. The mean reconstitution levels in mice transplanted with uncultured LSK cells were: 6 weeks, 50% (experiment 1), 35% (experiment 2), 17% (experiment 3), 40% (experiment 4); 4 months, 41% (experiment 1), 34% (experiment 2), 22% (experiment 3), and 40% (experiment 4). No differences in lineage distribution of donor-derived cells at 4 months were found between freshly isolated and cultured cells (see Table 2 for details).

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