American Society of Hematology

Fig. 5.

$Fig. 5. Ex vivo expansion of Lin−Sca-1+c-kit+ cells in FCS-containing cultures abrogates their ability for short- and long-term reconstitution of lethally irradiated recipients. / Five hundred freshly isolated LSK cells, or the expansion equivalent, subjected to indicated ex vivo expansion, were transplanted along with 150 000 or 200 000 unfractionated BM cells (Ly5.2+) into lethally irradiated (Ly5.2+) mice. (A) Total cellular expansion of LSK cells after 116 hours and 240 hours in serum-free and FCS-containing medium. After 6 weeks (B) and 4 months (C), peripheral blood cells from transplanted mice were analyzed for the presence of donor-derived (Ly5.1+) reconstitution. Data for 116-hour and 240-hour cultures are representative of 2 and 3 experiments, respectively. Data are presented as Ly5.1 reconstitution relative to the mean of that obtained with 500 freshly isolated LSK cells (mean, 17%-40% at 6 weeks and 22%-40% after 4 months). Numbers in parentheses show the total number of mice analyzed for each group of treatment.$

Ex vivo expansion of Lin⁻Sca-1⁺c-kit⁺ cells in FCS-containing cultures abrogates their ability for short- and long-term reconstitution of lethally irradiated recipients.

Five hundred freshly isolated LSK cells, or the expansion equivalent, subjected to indicated ex vivo expansion, were transplanted along with 150 000 or 200 000 unfractionated BM cells (Ly5.2⁺) into lethally irradiated (Ly5.2⁺) mice. (A) Total cellular expansion of LSK cells after 116 hours and 240 hours in serum-free and FCS-containing medium. After 6 weeks (B) and 4 months (C), peripheral blood cells from transplanted mice were analyzed for the presence of donor-derived (Ly5.1⁺) reconstitution. Data for 116-hour and 240-hour cultures are representative of 2 and 3 experiments, respectively. Data are presented as Ly5.1 reconstitution relative to the mean of that obtained with 500 freshly isolated LSK cells (mean, 17%-40% at 6 weeks and 22%-40% after 4 months). Numbers in parentheses show the total number of mice analyzed for each group of treatment.

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