Comparison of mature and primitive cell subsets constituting human fetal and CB-MNCs.

Representative analysis of cell surface markers using multiparameter flow cytometry of FB- and CB-MNCs. Composition of myeloid cells was indicated by CD33 and CD15 (A,B), B-lymphoid cells by CD19 and CD20 (C,D), T-lymphoid cells by CD4 and CD8 (E,F), and primitive subsets by CD34 and CD38 (G,H) cell surface expression. Average percentage for each subpopulation was calculated with SEM and indicated in each dot plot based on at least 4 independent FB and CB samples. Quadrant settings for each phenotype were based on isotype controls.