Comparison of engraftment composition in the BM of NOD/SCID mice transplanted with human fetal and CB stem cells.
BM from representative engrafted mice transplanted with 6 × 105 (2 FB-SRCs) or 20 × 105 (2 CB-SRCs) MNCs. Murine BM cells were stained with various human-specific monoclonal antibodies and analyzed by flow cytometry. (A,B) Histograms of CD45 (panleukocyte marker) expression indicating that 45% and 12% of the cells present in the murine BM were human from FB- and CB-transplanted animals, respectively. Analysis of lineage markers was done on cells within gate R1 and R2 (CD45+). (C,D) Representative isotype controls for nonspecific immunoglobulin G staining for each mouse. (E,F) Expression of myeloid marker CD33 and mature myeloid marker CD15, (G,H) pan–B-cell markers CD19 and CD20, and (I,J) CD38 and immature hematopoietic marker CD34. Multilineage engraftment shown here was similar to that found in additional mice transplanted with FB- and CB-MNCs and purified subsets of FB and CB CD34+CD38−Lin− cells.
