TSA induces apoptosis in ILT-Mat.

(A) Dose response of cell death in ILT-Mat cells by TSA. ILT-Mat cells were treated with TSA at the indicated concentrations (nmol/L) for 48 hours and investigated by trypan blue dye exclusion assay. The results, 4.3 ± 1.5(0), 24.6 ± 2.1(50), 51.7 ± 6.7(100), and 83.0 ± 7.6%(200) of total cells, are averages of triplicate samples from 3 experiments ± SD. (B) DNA fragmentation in ILT-Mat cells. Proliferating ILT-Mat cells were incubated with TSA at the indicated concentrations (nmol/L) for 48 hours. Low molecular DNAs were extracted from the treated and untreated cells and loaded onto 1.2% agarose gel with the size marker (λ phage/Hind III-digested DNA).