Effect of butyrate and TSA on IL-2-mediated gene expression in ILT-Mat cells.

(A) Butyrate and TSA abrogated the IL-2-mediated induction of c-myc, LC-PTP, and bag-1 genes. ILT-Mat cells were treated with 2 mmol/L butyrate (Bu) or 200 nmol/L TSA 0.5 hours prior to stimulation with IL-2. Following IL-2 stimulation for 1 or 6 hours, RNAs were extracted from the cells. Dose dependency for suppression of LC-PTP mRNA induction was analyzed (right panel). Cells were treated with 50 to 200 nmol/L TSA 0.5 hours prior to stimulation with IL-2 and incubated for 6 hours. (B) Butyrate and TSA did not affect stability of c-myc and LC-PTP (right panel) mRNAs. ILT-Mat cells were stimulated with IL-2 for 1 or 6 hours and thereafter treated with 20 μg/mL of actinomycin D with or without 200 nmol/L TSA or 2 mmol/L butyrate (Bu). RNAs were extracted from the cells at the times shown. RNAs were also extracted from the deprived cells as a control (C) and from the cells stimulated with IL-2 as a positive control (P). The 28S ribosomal RNAs are shown for the comparison of relative amounts of total RNA loaded.