Sodium butyrate and TSA induce apoptosis in F7 cells.

(A) Dose response of cell death in F7 cells by butyrate. F7 cells were treated with 1 to 4 mmol/L sodium butyrate for 48 hours and investigated by trypan blue dye exclusion assay. The results, 38.7 ± 7.0(1), 54.7 ± 7.6(2), and 67.3 ± 8.1%(4) of total cells, are averages of triplicate samples from 3 experiments ± SD. (B) F7 cells were treated with 25 to 100 nmol/L TSA for 48 hours and investigated by trypan blue dye exclusion assay. The results, 50.0 ± 4.0(25), 60.1 ± 3.9(50), and 91.7 ± 3.8%(100) of total cells, are averages of triplicate samples from 3 experiments ± SD. (C) DNA fragmentation in F7 cells. Proliferating F7 cells were incubated with sodium butyrate (Bu) or TSA at the indicated concentrations (mmol/L or nmol/L, respectively) for 48 hours. Genomic DNAs were extracted from the treated and untreated cells, and loaded onto 1.2% agarose gel with the size marker (λ phage/Hind III-digested DNA).