Effect of TSA on IL-2–mediated gene expression in F7 cells.

(A) TSA abrogated the IL-2–mediated induction of c-myc gene expression. F7 cells were treated with (+) or without (−) 200 nmol/L TSA or 100 μmol/L ara-C 0.5 hour prior to stimulation with IL-2 (+) for 1 hour. (B) TSA abrogated the IL-2–mediated LC-PTP gene expression. F7 cells were treated with 50 or 200 nmol/L TSA 0.5 hour prior to stimulation with IL-2 for 6 hours. RNAs were extracted from the treated cells and also from the cells which received no treatment as a negative control (C) and from the cells stimulated with IL-2 alone as a positive control (P). The 28S ribosomal RNAs are shown for the comparison of relative amounts of total RNA loaded.