Tissue expression mouse GPVI using ISH analysis.

(A) Day-12.5 mouse embryo stained with hematoxylin. (B) ISH of day-12.5 embryo using a GPVI probe. Hybridization is exclusively observed in the liver during embryogenesis. There was no signal seen with the sense probe (data not shown). High magnitude resolution shows that the only positive cell population corresponded to fetal megakaryocytes (data not shown). In adults, expression in the liver was no longer observed. (C) ISH of 6-week-old mouse femur with GPVI probe. Section shows expression restricted to megakaryocytes. Except for the spleen, there was no signal observed in any other adult tissues analyzed.