Fig. 4.
Identification of the protein encoded by human GPVI cDNA.
Proteins from platelets (1) or proteins from CHO cells transfected with an empty vector (2), the same vector containing human T268 cDNA (3), or the same vector containing human megakaryocytes (4) were separated by SDS-PAGE and blotted on PVDF membranes. The membranes were incubated (A) with 125I-Cvx, (B) with patient polyclonal anti-GPVI IgG antibody alone,8 or (C) in the presence of 0.5 μmol/L cold Cvx. 125I-Cvx was revealed by autoradiography. Anti-GPVI IgG was detected with peroxidase-coupled protein A and enhanced chemiluminescence.
