Fig. 7.
Fig. 7. Coexpression of rhusGPVI with FcR-γ chain. / Lysates from (A) platelets, (B) control, or (C) GPVI-transduced U937 cells were incubated with a polyclonal anti–FcR-γ antibody and protein A–sepharose. Immunoprecipitated proteins were separated by SDS-PAGE and blotted on a PVDF membrane. The membrane was incubated with a mixture of anti–FcR-γ and anti-GPVI antibodies revealed with peroxydase-coupled protein A and chemiluminescence.

Coexpression of rhusGPVI with FcR-γ chain.

Lysates from (A) platelets, (B) control, or (C) GPVI-transduced U937 cells were incubated with a polyclonal anti–FcR-γ antibody and protein A–sepharose. Immunoprecipitated proteins were separated by SDS-PAGE and blotted on a PVDF membrane. The membrane was incubated with a mixture of anti–FcR-γ and anti-GPVI antibodies revealed with peroxydase-coupled protein A and chemiluminescence.

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