TGF-β inhibits proliferation in M07 cells through an up-regulation of the CDK inhibitor p27.

(A) Reduction of the proportion of S phase cells in comparison to control after 48 hours of cultivation in either the presence of 10 ng/mL TGF-β and GM-CSF (100 U/mL) for M07 cells or with 10 ng/mL TGF-β for M07/p210 cells. Values reflect the mean ± SE of at least 3 independent determinations. (B) Levels of p27, cyclin E, and cyclin D3 in control (GM-CSF, 100 U/mL) and TGF-β (5 ng/mL)-treated M07 and M07/p210 cells after 18 hours. Protein expression was determined by means of Western blot analysis.