Cell-cycle analysis of Ba/F3-p185 cells transfected with Mig1-control or p27-Mig vector.

The subpopulation of highly EGFP-expressing cells was analyzed for cell cycle distribution. (A) Proportion of EGFP⁺ cells in S-phase transfected with p27-Mig in comparison to Mig-controls. Values reflect the mean ± SE of at least 4 independent analyses. (B) Representative result of cell cycle distribution in EGFP-expressing cells. Transfection with p27-Mig leads to a marked reduction of cells in S/G2M-phase (G1:64%, S:28%, G2/M:8%), whereas cells transfected with the Mig-control vector show a high cycling activity (G1:38%, S:46%, G2/M:16%).