Efficacy of 8 all-phosphodiester Mcl-1 AS oligodeoxynucleotides against Mcl-1 mRNA 2 hours after introduction of AS oligodeoxynucleotides.

U937 cells were treated for 3 hours with PMA before introduction of AS oligodexoynucleotides. Total RNA was extracted 2 hours after AS treatment, and Mcl-1 and actin mRNA levels were assessed by Northern blotting. The blots shown are representative of 3 independent experiments. Ethidium bromide–stained 28 and 18 S rRNA is shown to indicate the equivalence of loading. Significant difference from controls treated only with SL-O is indicated by a dagger (P ≤ .05) or an asterisk (P ≤ .01).