AS-fluorescein, annexinV-Cy3, and PI comprise the 3-color confocal microscopy of U937 cells treated with Mcl AS8 5.9.5 and Mcl invAS8 5.9.5 for 18 hours.

The cells were treated and cultured as described in “Materials and methods.” (A) Cells were treated with Mcl AS8 5.9.5 for 4 hours and (C) 18 hours. (B) Cells were treated with Mcl invAS8 5.9.5 for 4 hours and (D) 18 hours. (C and D) PI fluorescence is depicted in the top left panel, fluorescein in the top right, Cy3 in the bottom left, and a bright field superimposed with all fluorescence channels in the bottom right. (A and B) A bright field superimposed with all fluorescence channels. The results are representative of 3 separate experiments. Bar = 20 μmol/L.