Fig. 9.
Effect of monoclonal antibodies on the expression of GATA.
EMSA was performed using 1.5 μg of protein from Hep3B cells incubated with 10−4 mol/L L-NMMA under hypoxia (1% O2) for 1 hour; 2 μL of FCS (lane 3) or 0.5 or 1.0 μL monoclonal antibodies of hGATA-1 (lanes 4 and 5), hGATA-2 (lanes 6 and 7), and hGATA-3 (lanes 8 and 9) were incubated with nuclear extracts overnight at 4°C and then EMSA was performed. The dot at the left indicates the position of the respective proteins.
